PTSR on chlamydia: how to give a smear for analysis

Highly sensitive method for determining the organism of the causative agent of chlamydia - PCR on chlamydia. How to take this analysis and why? Among venereal diseases, characteristic for men and women, this is the most common infection. Timely delivery of the analysis allows you to identify infection and prevent complications.

What is it about

.

PCR (polymerase chain reaction) was invented in the 1970s. Improvements in technology have increased the accuracy of the result. The method is highly sensitive, therefore it will show the DNA of the pathogen even at small sample volumes. For this reason, PCR on chlamydia is preferable to antibodies. When conducting research on chlamydia, conditions are created artificially in which the infection multiplies, growing to volumes that allow a visual definition. The technique does not lose effectiveness even when the pathogen grows slowly.

Chlamydia trachomatis provoking chlamydia is common, but the course of the disease for a long time does not affect the quality of life, and changes do not notice. Those few signs that are manifested in slow processes, patients ignore or confuse with other disorders. PTSR on chlamydia precisely establishes, whether there was an infection and how much scale of defeat.

Material for the laboratory assistant - scraping, taken from the urethra, cervix. Hemotest, other variants of studies are accurate in 20% of cases. The correct result is obtained only by PCR.

.
.

Chlamydia: features

PCR as a method of diagnosing chlamydial infection has been common since the 90s. Specificity of technology in identifying the pathogen that lives inside another cell. But antibodies widely used in determining a number of diseases are not always able to detect the presence of intracellular infection.

Without treatment, chlamydia provokes secondary diseases of the female reproductive system, inability to bear fruit and conceive a child.

Chlamydia are parasites that are dependent on the respiration of host cells. Provoke development:

  1. genital infections;
  2. ornithosis;
  3. diseases of the respiratory system.

Chlamydia is common in humans and animals. For example, cats suffer from eye, gastrointestinal and lung. Diagnose with the same polymerase reaction.

Among people, the most common form is provoked by genital pathogens. Chlamydia provokes disorders of the urinary system, leads to the development of inflammatory processes in the pelvic cavity and the formation of adhesions.

Typical complications in women:

  • endocervicitis;
  • urethritis;
  • cervical erosion;
  • arthritis;
  • a suture process in the pelvic cavity;
  • ectopic pregnancy;
  • frequent miscarriages;
  • infertility.

Men are observed:

  1. cystitis;
  2. proctitis;
  3. prostatitis.

Diagnostics

Smear on chlamydia by PCR is an accurate way of determining if there is an agent in the body. Technology microscopic, involves the production of biological material and further reproduction in the laboratory.

Polymerase chain reaction is carried out when obtaining:

  • smear from the urethra;
  • prostate secretion;
  • smear from the cervix;
  • tissues of the vagina.

At home, the polymerase reaction is carried out using an express test. Acquire such in the pharmacy, biological material - urine. The reliability of this option is less than laboratory testing for chlamydia, but with a positive result there is no doubt - it's time to go to the doctor.

Before analysis

Preparation for the delivery of biomaterial - the guarantee of the accuracy of the result. Remember, although the method is effective, non-compliance with recommendations leads to an incorrect result.

Preparatory measures:

  1. 1-3 days before the delivery of biomaterials, sexual intercourse is excluded;
  2. the last urination takes place 3-4 hours before collection of material;
  3. analysis on chlamydia is given 3-4 days after the termination of menstrual bleeding;
  4. inadmissibility douching 24 hours before a visit to the doctor;
  5. Exclusion of medicines (primarily antibiotics), other than prescribed by the doctor, 7 days before the delivery of the biomaterial.

Peculiarities of polymerase chain reaction

Analysis of PCR on chlamydia in the laboratory is carried out on different biomaterials. If you used to use the tissues of the genital organs, the urethra, doctors now give an accurate diagnosis of saliva, semen, urine. The fence is made by a universal probe with the use of gynecological mirrors.

Taking the analysis for chlamydia by PCR, the working tool is placed in a protected container, filled with transport fluid, and sealed. Why is it necessary? The reaction is highly sensitive, specific and accurate, so the penetration of foreign bacteria, infections and viruses is unacceptable. When studying the materials obtained in the laboratory, a part of the DNA that is characteristic of chlamydia is isolated. The result is prepared in 6-8 hours. Accuracy and speed are guaranteed by the automation of the laboratory process.

Advantages of PCR on chlamydia

Choose a polymerase reaction due to:

  • the speed with which results are obtained;
  • the detection of latent infections;
  • universality;
  • sensitivity;
  • the possibility of detecting the DNA of the pathogen.

Pregnancy

Whether it is possible to pass or take place PTSR on a chlamydia at vynashivanii a fetus? How to take biomaterials in an "interesting position"? The technology coincides with that applicable for non-pregnant women. Doctors recommend taking a study at the planning stage of the child, but there are situations when the need arises after conception.

In pregnancy, analysis of chlamydia allows you to find out whether the risk of infection of the mother and the child is great. There is an opinion that the sampling of biomaterial damages the fetus. This is a stereotype that has no basis. A smear is received from the cervix. At the same time, the working tool of the gynecologist (probe, mirror) does not enter the uterus, does not harm the amniotic fluid. The process is safe, painless.

Benefits

In comparison with methods using antibodies, PCR indicates a variety of infections, including hidden, asymptomatic. The use of a polymerase reaction is diagnosed by:

  1. hepatitis B, C;
  2. chlamydia;
  3. mycoplasma genitalium;
  4. tuberculosis;
  5. HPV;
  6. herpes;
  7. Helicobacteriosis;
  8. mononucleosis of infectious origin;
  9. trichomoniasis.

results

PCR on chlamydia gives one of two results:

  • Negative when the material does not contain infectious traces.
  • Positive. It is possible even in the case where antibodies in other studies showed no pathogen. This is due to the sensitivity of the technology. This result indicates the presence of infection in the cells studied. The result is qualitative, the number of conclusions can not be made.

If the result is positive, the doctor determines how to clarify the diagnosis and what treatment is required.

Conducting PCR

Material for the laboratory is obtained with a plastic probe or gynecological brush. The obtained substances are collected in a conical sealed 1.5-gallon tube, fill the unoccupied space with a pinkish medium.

Test tubes are disposable, sterile. Materials for investigation on chlamydia are obtained from the urethra, cervix. It is unacceptable that the smear contain blood, so scraping is taken carefully.

The study of the biomaterial is carried out by amplification. The process gives an accurate result in 6-8 hours. In this case, the DNA sequence is copied due to the DNA polymerase. At each cycle, a primer is introduced. The cycles are replaced by a temperature regime.

High sensitivity is the advantage of PCR on chlamydia in comparison with ELISA and immunofluorescence study.

The reaction occurs in three stages:

  1. Increasing the temperature to 94 ° C transforms the DNA molecule into single-stranded (denaturation);
  2. short synthetic fragments bind to the given sections of protein chains (annealing);
  3. new DNA is synthesized when heated to 70-72 ° C (polymerization).

Accuracy is guaranteed by the exclusion of cross reactions.

Summing up

Despite the prevalence and danger of chlamydia, PCR reveals the disease in an active and latent form. It is recommended to do research at the first symptoms of infection and after treatment of the disease.

Do not hesitate to go to the doctor, having suspected sexual infections! For a woman, especially for a future mother, it is important to monitor your health and promptly contact the clinic. Remember, neglected chlamydia leads to serious organ damage and infertility. Having analyzed the PCR method, the woman not only ascertains the presence of chlamydia in the body, but also receives accurate data on other pathogens, including hidden ones.